8 Tips for Effective Revision

8 Tips for Effective Revision 8 Tips for Effective Revision If youve got exams coming up, youll probably be hard at work revising. But how can you ensure that your time spent revising is worthwhile? Weve prepared our top 8 revision tips to help you get in shape for those tests! 1. Start with Good Notes Ok, so this might not help you now, but its definitely something you can do going forwards. Make sure you take clear, legible notes in class so that when you come back to them they make sense! Try highlighting them with a color-coding system as part of your revision. It really helps you to make sense of them. 2. Make a Revision Timetable If youve got several tests to revise for, it might be helpful to make a timetable so that you can make sure you are spending enough time on each subject. Stick it up on your wall and try to follow it. 3. Take a Break When making your timetable, make sure you add in breaks. Ideally, you should have a 5-minute break once every half hour. Get up, stretch, have a cup of coffee, or go for a walk outside. Taking regular breaks will help your brain to function more effectively. 4. Sort Out your Revision Space The first thing you should do before revising is to tidy your revision space. If it is clear and uncluttered, you will find it much easier to work. 5. Dont Memorize, Summarize! Dont just re-read your notes, try making a summary of them in your own words. This will help you to remember them as you will have to process the words rather than simply reading them. Its surprising how much you can read whilst daydreaming! 6. Use Past Papers Your course tutor will probably be able to provide you with some past papers if you ask. There might even be some available on the Internet. Make sure you time yourself and use proper exam conditions. 7. Use Visual Aids If you have a visual brain, you might find it helpful to create charts or infographics. Doodling the main facts out can be a great way of making summaries of your notes. The funnier these are, the more you should remember the information! 8. Eat, Sleep, Run! Dont forget that your body needs fuel, rest, and exercise for your brain to function well. Dont be tempted to cram all night before a test. Plan out your revision for a few weeks before the test and dont do any revision at all the day before. Instead, go for a swim, eat well and go to bed early. Have a banana and plenty of water for breakfast and your brain will be on top form!

General Hummel

In the rock it is a very powerful and shows a lot of the different types of leaderships style that we had talked about in the past. With the military mercenary group in my opinion yes there is a commander General Hummel but what I noticed as I was watching the movie was that all of them respected and followed his orders but when it came to getting down there was a change. First lets go over the four main men with in the group and look at each on of their type of leadership demonstrated in the movie. Major Baxton, General Hummels right hand man. He demonstrates some what of a pleaser because he follows orders and wants to do right by his commander the General, also of an achiever he wants to get things done right the first time around he is very goal oriented when he is told do something thats what he sets out to do. Captain Hendrix on the other hand shows to be more of an attacker than anything else. He has a bad attitude he is very much in control of himself and when h! e says jump that what he wants to happen. He is also an achiever goal oriented he is very self confident in what he sets out to achieve. Captain Frye and Capt Darryl are performers and attackers. They both they have no feeling but are both very strong willed individuals. They have orders to follow and they see that they are achieved no matter how they need to do it. If its hurting another person so be it. They had an order and they will follow through till the end. Gunner Sgt Crisps is an avoider and a pleaser. There is a scene where the Capt. Hendricks, Capt. Fry and Capt. Darryl are confronting General Hummel on a situation and told Crisp to apprehend the General and he doesnt want to do it because Hummel is his general but is ordered by the three individuals to do so he realizes that Hummel is no longer in control and to keep from upsetting the other Capt. he does it. He is very quit and to himself. Follows orders and does what h..

Fashion and consumer culture Essay Example | Topics and Well Written Essays - 3250 words

Fashion and consumer culture - Essay Example The essay "Fashion and consumer culture" discusses the role of consumer goods and services. According to the domain of social psychology, fashion products represent symbolic attributes that speak volumes about an individual’s position in life in regards to income, trend-consciousness, and social group membership. It is the symbolism projected by fashion attire that largely dictates the level to which an individual moulds their lifestyle against social group affiliation needs. On the other hand, the question as to whether fashion is a symbolic methodology to ensure a degree of individualism is raised. Symbolic purchases represent a status representation reflecting socio-economic background or peer group affiliation, thus theoretically it is the social environment that determines fashion consumption. Is fashion consumption solely based on social attachment or is fashion a break from pre-established social norms to project individual innovation and creativity as the dominant reas on for consumption? However, research identifies that fashion consumption is more closely related to social affiliation, an assumption based on characteristics in the real-world fashion marketplace that point toward the psychological mechanisms of social group identity. Based on differing viewpoints, the question is raised as to whether fashion consumption is solely individualistic or based on social factors that influence buying behaviours. This paper explores how either individualism or social factors influence social lives.

Rasputin and the fall of the Romanovs Term Paper

Rasputin and the fall of the Romanovs - Term Paper Example Rasputin played a very significant part in the fall of the Romanovs. Rasputin and the fall of the Romanovs Introduction Rasputin could have done better to prevent the fall of the Romanovs. The research delves on Rasputin’s role in the downfall of the Romanovs. The research delves on role of Rasputin in preventing the people’s uprising. Rasputin was instrumental in the fall of the Romanovs. Gregory Rasputin had exercised significant influence on the Russian Tsar’s family (Thompson, 1991). However, Rasputin was not the only individual who exercised a huge influence on the Russian Tsar’s family. Prior to Rasputin, there were other individuals who had exercised a similar or even greater hold on the Tsar’s family. Most Russian villages had their own share of holy fools. For hundreds of years, the Russian villages had to contend with the different holy fools of their own. During other time periods, the holy fools were sometimes naked. On other occasions, the holy fools were described as unwashed. Sometimes, the holy fools were dirty. Further, another group of holy fools used queer attires. All the holy fools had one very similar trait. The similar trait is that they literally wreaked havoc on the community’s once peaceful environment. The holy fools exercised religious influence on the affected community. As expected, the pronouncements of the holy fools triggered many controversies. Some of the holy fools espoused premarital sex. History shows that the holy fools belonged to a long line of Russian Shamans. The Shamans used many decorations to improve their physical appearances. The Shamans used metal and other accessories in order to attract attention (Thompson, 1991). As expected, the Holy fool concept creates an impression where Russian government’s leadership is excellent. The Holy fool concept espoused by Rasputin triggered the expected collapse of the Tsar government. The Holy fool concept indicated that the peop le on the outskirts of the Tsar’s Russian community. The Holy fool, Rasputin, was able to create a false impression that there are rumors A group of rebels were about to stage several attacks on the Russian Tsar’s Kingdom. The attacks would initially crop up in the Russian countryside (Thompson, 1991). As expected, the religious leaders of the Orthodox religion focused on ejecting the holy fools from Russian society. The religious leaders felt that excess efforts are needed to prevent the growing spread of the holy fools’ false teachings. Divulging the false teachings of the holy fools would benefit the Russian church leaders. The divulging would prevent the holy fools from injecting false doctrines on the unsuspecting and confused Russian public. One of the victims of the holy fools is Russian Tsar’s family (Thompson, 1991). Further, it is very clear that Rasputin, a holy fool, was instrumental in sparking the downfall of the Russian Tsar Nicholasâ€℠¢ empire (Sarah, 2010). The Tsar had intended to give his throne to his brother. The Tsar could not give the Russian empire’s throne to his son on the ground of fitness. The son’s sickly and weak physical condition could hinder a normal management of the huge Russian government. However, The Tsar’s brother refused to accept the Tsar’s juicy offer. With no one eager or able royal family member eager to receive the Russian throne, the Tsar’s time as ruler of Russia was slowly being trimmed

Close reading Essay Example | Topics and Well Written Essays - 1250 words

Close reading - Essay Example Matusky and Sooi (2004) studied the Malaysian dance and commented that the Malaysian musical traditions were always accompanied with dance or dance drama that did not have any dialogue as its essence was to engage the audience to the set act so that they could derive a meaning out of the performance. This acted as an act that included the attributes of the classical or folk music2. As a result, the dance incorporated the use of the body more so the hands and the feet. Thus through their traditional Malay music, the Malay dancer(s) introduced the use of hands as they swayed their hands from side to side or up and down as a form to express emotion and attachment to the song. Malay classical dance history and characteristics The early Malay dances incorporated various dance forms such as mak yong, mek mulung and manohra and these were practiced often in the courts thus their origination. The mak yong comprised of a much dramatized dance act that depicted the stories of the princes and p rincesses. The women who acted as princesses wore royal costumes while the men wore the same costumes and an additional male clown. Their dance was also accompanied by drums, gongs and a serunai. The dance is also related to Puteri, which is an ancient ritual that was believed, that when it was performed by the dancers, it was prone to release supernatural powers and as a result, the kings did not follow much on that, rather they adopted the dance that was related to theatrical performances. This dance involved the simple act of a female and a male at the courts. Manohra also shares some aspects with mak yong such as the dance that consists of the female and the male and it also incorporates more of dance rather than the story that is behind to the dance set being performed. Manohra also served as a traditional and respected ritual and it was also believed to have the aspect of super natural powers. When the dance was performed, it was always accompanied with the serunai, two gedung , two gedumbak, kesi, bamboo or wood clappers and gongs. As of today, there exists only two active manohra groups’ in Malaysia and tey happen to occupy the parts of Kelantan. Mek mulung on the other hand involved a dance drama that depicted a local legend that had happened in the community. As a result this dance also had the same aspects in meaning and body gesture when compared to mak yong and manohra. When the dance was performed, it was always accompanied with the use of the serembong, gong, serunai and ceruk. The oldest surviving Malaysian performance traditions can be dated back to the peoples of the Orang Asli communities of Peninsular Malaysia who are very scarce in numbers in the region. Some of the community sub groups include the Negrito communities that include the Bateq, Jahai, Kensui, Kintak, Lanoh and Mendriq peoples and they are located in the Kelantan, Pahang, Perak and Terengganu regions. The Senoi include the Che Wong, Jahut, Mahmeri, Semai, Semoq Beri and Temiar peoples who are located in Kelantan, Pahang, Perak and Selangor, and the Proto-Malay peoples incorporate the Jakun, Orang Kanaq, Orang Laut, Orang Seletar, Semelai and Temuan) majoritively in Johor, Melaka, Negeri Sembilan and Pahang. The Orang Asli performances involved the incorporation of music, song and dance and they were preformed mainly at social events

GFP Practical Report

GFP Practical Report GFP is very useful as a reporter protein. After its discovery in 1962 its practical applications were put into use 30 years later by adding the coding DNA of GFP before the stop codon of other proteins. This allows for an easily detectable marker of the proteins presence without needing additional cofactors or causing any harm to the organism. The spectral characteristics of GFP can be changed by making mutations to the protein. In this investigation a Y66W mutation was made to wildtype GFP in order to produce a shorter excitation and emission wavelength. The mutation was made using QuikChange site directed mutagenesis. The protein was then cloned into BL21(DE3) pLysS for expression. The cells were then lysed and applied to a Ni-NTA column. This fractionated the lysate in order to analyse these fractions using SDS-PAGE, fluorescence and Bradford assays. It was found that the Y66W mutation was successfully added but due to another mutation in the stop codon additional amino acids were added to the C terminus of the protein. It was also found that purification was partially successful as GFP was eluted in the correct fraction. This is supported by the Bradford and fluorescence assays. The green fluorescent protein (GFP) is a 238 amino acid protein with a molecular mass of 26,870 Da. It was first isolated from the jellyfish species Aequorea Victoria by Osamu Shimomura in 1962 (1). GFP is expressed in small photoorgans that are situated in the umbrella of the jellyfish. Douglas Prasher first realised the potential of GFP as a reporter protein (2). As proteins are smaller than the resolving power of electron microscopes, Prasher thought the GFP gene could be added into the gene for haemoglobin before the stop codon. This would allow the protein of interest to maintain all of its functions but would have the GFP protein at its C terminal end. This means that detection of GFP fluorescence would also indicate the presence of haemoglobin. Furthermore, GFP does not require additional cofactors or substrates to fluoresce. This means that it works extremely well as a non-invasive method of detection of protein expression. GFP is also non-toxic so it is able to be used in vi vo without causing damage or harm to the organism. Crystallisation studies (3) have shown that GFP has a barrel structure with the chromophore buried in the centre. This chromophore is comprised of 3 amino acids (Ser 65-Tyr 66-Gly 67) that undergo a series of spontaneous cyclisation reactions to create the active chromophore. Wild type GFP has a major excitation peak at 395 nm and a minor one at 475 nm with an emission peak at 509 nm. In vivo GFP is coupled to the protein aequorin which induces a blue glow when it interacts with Ca2+ ions and breaks down luciferin. This light is able to excite GFP and cause fluorescence. In vitro this is not the case, however GFP fluorescence can be easily induced by irradiating GFP with UV light. As with all proteins, GFP can be mutated. By mutating key residues, such as residues in the chromophore, it is possible to change the characteristics of GFPs fluorescence. The first of many mutations was the S65T mutation (4). This mutation improved the characteristics of the protein including increased photostability, fluorescence and a shift of the major excitation peak. This investigation is based on the engineering of GFP to create a mutant of GFP with a shorter excitation and emission wavelength by inducing the Y66W mutation. The aims of this investigation were as follows. To carry out site directed mutagenesis of GFPuv to clone into pET28c and transform the products into XL-1 super competent cells. Extraction of the plasmid after incubation overnight to check the purity and concentration of DNA. Preparation and transformation of BL21(DE3) cells. Lyse these cells and fractionate the lysate to purify his tagged GFP using a Ni-NTA column. Finally, detection of purified GFP by SDS- PAGE, Bradford assay and fluorescence. The workflow of the investigation can be found in figure 1 in appendix 1. A more detailed protocol can be found in the BIOC2302 semester 2 practical manual on pages 6-15 with rationale for all experiments. In site directed mutagenesis I 31 ÃŽ ¼l of water was added to the PCR reaction to give a total reaction volume of 50 ÃŽ ¼l. In site directed mutagenesis II a supplied culture of cells was used in the experiment rather than cells from the transformation colonies in site directed mutagenesis I. His tagged GFP was used instead of the mutant in the protein purification experiment in order for easier administration as the process is the same. Site directed mutagenesis I Before the wet lab work began it was first necessary to design primers for QuikChange to induce the Y66W mutation into the wild type GFP. These can be seen as figure 2 in appendix 2. These were created using the QuikChange primer design tool on the Agilent website. The site directed mutagenesis was carried out using the primers supplied to induce the correct mutation. The products of this were cloned into the pET28c plasmid and the XL-1 super competent cells. The cells were plated as per the BIOC2302 practical manual and left to incubate overnight. Site directed mutagenesis II Upon checking the plates in the next session it was found that no transformed colonies had grown so a new culture was supplied. The undigested plasmid control grew approximately 50 colonies The culture of BL21(DE3)pLysS cells was set up and the OD600 were recorded. They can be seen in table 1 in appendix 3. Within 50 minutes the culture had reached an OD600 of 0.483 meaning the cells were at the correct density for lysis. The cells were prepared as per the BIOC2302 practical manual and the recombinant plasmid was extracted. The concentration measured was 121.7 ng/ÃŽ ¼l and the A260/A280 was 1.86 using nanodrop. Therefore, the ethanol precipitation was not carried out. To prepare for sequencing 4.11 ÃŽ ¼l of this solution was diluted, with 5.98 ÃŽ ¼l EB buffer, to the correct concentration. This was then sent to be sequenced, the results of which can be seen in appendix 4. The primer has been highlighted in green and is surrounded by a box with the mutated codon in red. A deletion also occurred in the stop codon of the mutant as highlighted by the second box with deleted bases highlighted in blue. Protein purification The plates were inspected in the next session. It was found that the 200 ÃŽ ¼l transformation plate grew 3 colonies and the 50 ÃŽ ¼l transformation plate grew none. Transformation efficiency can be calculated for the 200 ÃŽ ¼l plate as 37 transformants/ÃŽ ¼g of DNA. The cells were weighed and found to be 0.539 g so 2 ml BugbusterTM used. After lysis and fractionation the SDS-PAGE samples of each fraction were prepared and loaded onto the gel. The Bradford assay was carried out while the gel ran.   The BSA standards were calculated and the contents of each standard well can be seen in table 2 in appendix 3. The fractions were then diluted into their wells and the contents can be seen in table 3 in appendix 3. The plate was filled according to the map in figure 2 in appendix 5. The plate was ran and the absorbances for the BSA standards were taken from the plate readout and inputted into table 4 in appendix 5. From here a calibration graph was set up using GraphPad Prism and can be seen as graph 1 in appendix 6. This graph shows that the data points for the standards do not fall near the line of best fit. The absorbance results from the plate readout for all of the fractions were imputed into table 5 in appendix 7. The equation of the line from graph 1 was then used to calculate the concentration of protein in each of the fractions. All of these values were also inputted into table 5. With the Bradford assay complete the SDS-PAGE gel was disassembled, stained and a picture was taken. A map of the gel can be seen as figure 3 in appendix 7 and the picture of the gel can be seen as figure 4 in appendix 8. By looking at the picture it can be seen that in lanes 2, 3, 4 and 9 there are dark bands spanning the entire lane. In 5, 6 and 8 there is faint banding across the well. In well 7 there is a distinct small band in between the 25 kDa and 37 kDa molecular markers. Lane 8 shows no bands at all. Finally the fluorescence assay was carried out as per the map of the microtiter plate in figure 5 in appendix 7. The results from the plate readout were inputted into table 5. From here a graph comparing the log of protein concentration compared to fluorescence of each fraction was plotted and can be found as graph 2 in appendix 6. This shows elution 1 with the highest fluorescence and the unbound x10 had the lowest. However, when comparing protein concentration the unbound fraction had the highest and wash 2 had none. Percentage fluorescence was also calculated and inputted into table 6 in appendix 9. The first aim of this experiment was to transform the site directed mutagenesis products into XL-1 super competent cells. The correct primers were used in order to induce the Y66W mutation into the parental DNA. However, no colonies that were meant to take up the mutated plasmid grew but the undigested control grew around 50 colonies. This means the cells did not take up the plasmid because otherwise they would have grown on the plate. This could be due to a mistake made in making the PCR reaction mixture or the DNA may have become damaged at some point in the experiment. Additionally, the suppliers of the XL-1 super competent cells advice to avoid large changes in temperature. This was unavoidable in this experiment and may have contributed to the cells not taking up the plasmid. In the future more care should be taken while plating and preparing the cells. Also preparation of any reaction mixtures should be checked very closely in order to ensure the correct reactants are added in the correct amounts. In site directed mutagenesis II the cell culture was lysed when the OD600 was 0.483. That is because E.coli cells are most likely to be made competent when they enter early log phase. This corresponds with an OD600 of 0.4-0.5. The DNA concentration extracted in this experiment was found to be 121.7 ng/ÃŽ ¼l and an A260/A280 of 1.86. This means that the DNA is good quality as the desirable range for A260/A280 is 1.7-2.0 and the concentration was much higher that what was required. However, in future experiments to test for reliability multiple results should be taken. Furthermore, the data could have been confirmed by using the spectrophotometric method alongside using nanodrop. The sequencing results in appendix 4 confirmed the successful incorporation of the Y66W mutation into GFP, creating the CFP mutant. However, the second mutation at the stop codon deleted 2 bases including the first base of the stop codon. This means that when the protein is expressed the ribosome will not stop and instead will continue to add amino acids onto the C terminus of the mutant until it reaches a new stop codon. There 144 bases between the original stop codon and the next in frame stop codon meaning 48 additional amino acids will be added to the C terminus. This codon can be seen highlighted in purple below the original stop codon. These additional amino acids could affect the folding or could increase the likelihood of aggregation of the mutant protein. In the protein purification experiment the 200 ÃŽ ¼l transformation plate grew 3 colonies and the 50 ÃŽ ¼l transformation plate grew none. The transformation efficiency on the 200 ÃŽ ¼l plate was 37 transformants/ÃŽ ¼g of DNA. The reason why this is so low could be due to a number of factors such as the plating technique or the cells may not have been left to chill on ice for the optimum amount of time. However, the negative control did not grow any colonies, confirming that all of the bacteria on the transformation plate were transformed. Again, more steps should be taken if this was to be carried out again to ensure that proper plating and prepping protocol is followed. The Bradford assay shows that in wash 2 there was no protein in the well. This means that any protein found in elutions 1 and 2 should all be His tagged GFP that bound the Ni-NTA column. This can be confirmed by the fluorescence results as the elution 1 fraction contained the majority of the total fluorescence with 44.13% of the total. However, all other fractions also produce some fluorescence. This could be due to GFP contamination in the other fractions. This could have occurred due to the resin being saturated, preventing further binding to the column. It could also be due to aggregation of the protein obscuring the His tag and preventing binding. Furthermore, the plots on the calibration graph do not fall on the line of best fit. This means that the equation of the line is not accurate and protein concentrations calculated using it are also inaccurate. Therefore, there could be more protein in each fraction than was calculated. This could account for the fluorescence in the wash and unbound fractions. The Bradford assay is quite limiting. This is due to the fact the assay only measures protein concentration rather than GFP concentration. This means that it is unsure whether the protein concentration measured in elution 1 and 2 is all GFP or it is contaminant protein. The same can be said for the other fractions, its unsure whether the protein concentration measured has been contaminated by GFP. In the future this assay should be carried out again to try and reduce contamination. The calibration graph should also be repeated until all of the data points fall on the line of best fit. Otherwise none of the calculated protein concentrations are accurate. Finally, the SDS-PAGE results shows banding in wash 1, 2 and elution 1 and 2. This suggests that there is contaminating protein in all of these fractions. Elution 1 shows a clear band at approximately the 26-27 kDa mark as it is present just above the 25 kDa marker and is well below the 37 kDa marker. This suggest the band in elution 1 is GFP as it is the appropriate size and is in the expected fraction. Another source of error could be due to the amount of pressure applied to the pipette. This will vary from person to person and will affect the volume of the solution being pipetted. As such small volumes were being used and there was a lot of solutions to be pipetted it is very possible a mistake was made. This mistake would have a big effect on the concentration and therefore could have a big effect on the absorbance values. These errors can be avoided in future by using the appropriate pipette for the volumes being used. Further reduction in errors can come from correct technique and by doing replicates and averaging values. There could be some error in the microtiter itself. There may have been markings or scratches on the plate that werent seen at the time. This could affect how the light passed through the reader and therefore affect the absorbance values. In conclusion, the aims of this investigation were to induce the Y66W mutation into wild type GFP using QuikChange site directed mutagenesis. Furthermore, the protein was to be expressed in competent BL21(DE3) pLysS cells. Finally wildtype GFP was to be purified using a Ni-NTA column and the fractions analysed with SDS-PAGE, fluorescence and Bradford assays. The investigation successfully introduced the Y66W mutation into wildtype GFP. However, the stop codon was also mutated adding an extra 48 amino acids on the C terminal of the protein.   A band indicating the presence of GFP was found at the 26.9 kDa mark in elution 1, indicating it was bound to the column and was eluted. However, all factions were contaminated with other protein. References      Ã‚   1. Shimomura, O., Johnson, F., and Saiga, Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous. s.l. : J. Cell. Comp. Physiol. 59:223-39, 1962. 2. Prasher, D., Eckenrode, V., Ward, W., Prendergast, F., and Cormier, M. Primary structure of the Aequorea Victoria green-fluorescent protein. s.l. : Gene 111 (2)229-33, 1992. 3. Ormo, M., Cubitt, A., Kallio, K., Gross, L., Tsien, R., and Remington. S. Crystal structure of the Aequorea Victoria green fluorescent protein. s.l. : Science 273:1392-5, 1996. 4. Heim R, Cubitt AB, Tsien RY. Improved green fluroescence. s.l. : Nature. 373 (6516): 663-4., 1995.

The Witching Hour :: essays research papers

The Witching Hour Title: The Witching Hour Author: Anne Rice Copyright Date: 1990 Number of Page Read: 1043 Three main elements classify the genre: Gothic, Mystery, and Romance. I classify Gothic novels as stories pertaining to a dismal atmosphere, such as Edgar Allan Poe's literature. . Lovers who unlock thirteen generation's of Mayfair family secrets and incest; discovering that their intervention becomes a more complex-intertwining destiny. Our Antagonist Michael Curry, a 48-year old Irish man who had lost himself in a world in which he had accomplished his dreams, experienced his emotional pain, and yet he felt empty. Michael approached the rocks of the bay, thought of his life and emptiness, then he slipped and fell to his fatal doom in the sea. Found by a woman known as Rowan Mayfair , he discovered that he was dead for over an hour as he rested in the hospital. Michael also discovered that he had received the gift of seeing images by using his hands to touch objects, and that he chose to come back. He was burdened by the images and the vision after his death, that he had a purpose, that he was sent for a reason. Something that had to do with a doorway, and the number thirteen. After isolation from the press of the burden of his powers, he found himself wanting to go back on the deck of the boat where he was rescued. He wanted to talk to the woman who rescued him, for he thought that she would let him touch the boat to recover images that night. He discovered that this neurosurgeon, Dr. Rowan Mayfair, was the veritable love of his life. After he discovered how much he deeply loved Rowan, he began to reminisce the images of his purpose; that certain elements and images of his childhood hinted him to return to the house he had been fascinated with in New Orleans as a child. Another major character of the story, Englishmen Aaron Lightner, was a part of an archaic organization known as the "Talamasca". Aaron studied a family called the Mayfairs in New Orleans, because his organization had believed the Mayfairs to be witches. He devoted his life to the history of the Mayfair witches, and his organization had compiled a history of the Mayfairs since the days of the 17th century inquisition in Europe. As he watched Michael and Rowan from the corners of darkness, he intervened their lives to enlighten their knowledge of Rowan's family history—and its dangerous potential. Rowan did not know a single thing of her family history, as she was left in the dark her whole life by her aunt